TAK1-TAB1 Fusion Protein, Active

N-terminal His6-tagged, recombinant, human TAK1 amino acids 1-303, fused to human TAB1 amino acids 437-end, expressed by baculovirus in Sf21 insect cells. ||Specific Activity: |~50U/mg ||Unit Definition:|One unit of TAK1-TAB1 fusion activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100uM.||Applications:|Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested.||Recommended Dilution: |MS Tryptic Fingerprint: Confirmed identity with the translated sequence listed in the protocal.|Kinase Assay: 19-48ng phosphorylated 2mg/ml casein.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Handling Recommendations:|Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.