Svbp (NM_024462) Mouse Tagged ORF Clone
Cat# MR200054
Size : 10ug
| Product Data | |
| Target Symbol | Svbp |
|---|---|
| Synonyms | 2410005K17Rik; AI851162; Ccdc23 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MR200054 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCACCTGCCCGGAAAGAAAAATCCAAAGTTAAAGAACCAGCCTTCAGAGTGGAGAAGGCTAAGC AGAAATCTGCCCAGCAGGAGCTGAAGCAAAGACAGAGAGCAGAGATCTATGCTCTCAACAGAGTCATGAC GGAGCTGGAGCAGCAGCAGTTTGATGAGTTCTGTAAGCAGATGCAGCCGCCTGGGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR200054 protein sequence Red=Cloning site Green=Tags(s) MDPPARKEKSKVKEPAFRVEKAKQKSAQQELKQRQRAEIYALNRVMTELEQQQFDEFCKQMQPPGE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_024462 |
| ORF Size | 201 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024462.2, NP_077782.1 |
| RefSeq Size | 748 bp |
| RefSeq ORF | 201 bp |
| Locus ID | 69216 |
| UniProt ID | Q99LQ4 |
| Cytogenetics | 4 D2.1 |
| MW | 7.8 kDa |
| Summary | Enhances the tyrosine carboxypeptidase activity of VASH1 and VASH2, thereby promoting the removal of the C-terminal tyrosine residue of alpha-tubulin (PubMed:29146868). Also required to enhance the solubility and secretion of VASH1 and VASH2 (By similarity).[UniProtKB/Swiss-Prot Function] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| MC200194 | Ccdc23 (untagged) - Mouse coiled-coil domain containing 23 (Ccdc23), transcript variant 2, (10ug) | 10 ug | | |
| MR200054L3 | Lenti ORF clone of Ccdc23 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 23 (Ccdc23), transcript variant 2 | 10 ug | | |
| MR200054L4 | Lenti ORF clone of Ccdc23 (mGFP-tagged) - Mouse coiled-coil domain containing 23 (Ccdc23), transcript variant 2 | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.