Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western blot analysis of SOX2 in mouse brain lysate with SOX2 polyclonal antibody at 0.5 ug/mL (Cat # PAB12025).

View product citations for antibody PAB12025 on CiteAb
  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of SOX2.PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide corresponding to amino acids 1-100 of human SOX2.

    Host

    Rabbit

    Reactivity

    Chicken, Human, Mouse, Sheep, Xenopus, Zebra fish

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    Flow Cytometry (1:100)
    Immunocytochemistry (1:125-1:250)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:125-1:250)
    Western Blot (0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In Tris-glycine, 150 mM NaCl (0.05% sodium azide)

    Storage Instruction

    Store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western blot analysis of SOX2 in mouse brain lysate with SOX2 polyclonal antibody at 0.5 ug/mL (Cat # PAB12025).

    Immunohistochemistry

  • Gene Info — SOX2

    Entrez GeneID

    6657

    Protein Accession#

    P48431

    Gene Name

    SOX2

    Gene Alias

    ANOP3, MCOPS3, MGC2413

    Gene Description

    SRY (sex determining region Y)-box 2

    Omim ID

    184429 206900

    Gene Ontology

    Hyperlink

    Gene Summary

    This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq

    Other Designations

    SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2

  • Interactomes
  • Diseases