Images
Western Blot (Tissue lysate)
Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Western Blot (Tissue lysate)

Western blot analysis of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:1000 dilution.
Lane 1: Cortex
Lane 2: Cortex X Medulla

Immunofluorescence
Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Immunofluorescence

Immunofluorescence staining of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:200 dilution, DAPI merged.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against recombinant SLC12A3.

    Immunogen

    A synthetic peptide corresponding to amino acids 74-95 at N-terminus of rat SLC12A3.

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Specificity

    1 ug/mL of PAB28749 was sufficient for detection of SLC12A3 ,~160kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Form

    Liquid

    Conjugation

    ATTO 390

    Purification

    Protein A purification

    Recommend Usage

    Immunocytochemistry/Immunofluorescence
    Immunohistochemistry (1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Application Data with Unconjugated Antibody

    Western Blot (Tissue lysate)

    Western blot analysis of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:1000 dilution.
    Lane 1: Cortex
    Lane 2: Cortex X Medulla

    Immunofluorescence

    Immunofluorescence staining of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:200 dilution, DAPI merged.

  • Applications

    Western Blot

    Immunohistochemistry

    Immunofluorescence

  • Gene Info — SLC12A3

    Entrez GeneID

    6559

    Protein Accession#

    NP_000330; NP_062218; P55018; P55017

    Gene Name

    SLC12A3

    Gene Alias

    FLJ96318, NCCT, TSC

    Gene Description

    solute carrier family 12 (sodium/chloride transporters), member 3

    Omim ID

    263800 600968

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    Na-Cl symporter|NaCl electroneutral thiazide-sensitive cotransporter|sodium-chloride cotransporter, thiazide-sensitive|solute carrier family 12, member 3|thiazide-sensitive Na-Cl cotransporter

  • Gene Info — Slc12a3

    Entrez GeneID

    54300

    Protein Accession#

    NP_000330; NP_062218; P55018; P55017

    Gene Name

    Slc12a3

    Gene Alias

    NCC, TSC

    Gene Description

    solute carrier family 12 (sodium/chloride transporters), member 3

    Gene Ontology

    Hyperlink

    Gene Summary

    O

    Other Designations

    solute carrier family 12, member 3|thiazide-sensitive Na-Cl cotransporter

  • Interactomes
  • Diseases