Sirt1 Mouse siRNA Oligo Duplex (Locus ID 93759)
Cat# SR427400
Size : 1kit
Sirt1 Mouse siRNA Oligo Duplex (Locus ID 93759)
SKU
SR427400
Sirt1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
In Stock*
| Product Data | |||||||
| Purity | HPLC purified | ||||||
|---|---|---|---|---|---|---|---|
| Quality Control | Tested by ESI-MS | ||||||
| Sequences | Available with shipment | ||||||
| Sequences | Available with shipment | ||||||
| Components | Sirt1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 93759)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml Once you identified the best-performing siRNA sequence of the kit, you can order a single siRNA individually.
If you need negative control, please order SR30004 (2 nmol of scramble siRNA control) | ||||||
| Stability | One year from date of shipment when stored at -20°C. | ||||||
| # of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). | ||||||
| Note | Single siRNA duplex (10nmol) can be ordered. | ||||||
| Reference Data | |||||||
| RefSeq | NM_001159589, NM_001159590, NM_019812 | ||||||
| UniProt ID | Q923E4 | ||||||
| Synonyms | AA673258; S; Si; Sir2; Sir2a; Sir2alpha; SIR2L1 | ||||||
| Summary | This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] | ||||||
| Performance Guaranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at tech@biotrend-usa.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). | ||||||
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.