Reagents and instruments for immunology, cell biology and molecular biology.

Sheep Interleukin 4, IL-4 ELISA Kit

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Cat# E0079Sh-48wells

Size : 48wells

Brand : Bioassay Technology Laboratory

Sheep Interleukin 4, IL-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 0.53ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Interleukin 4

Code

E0079Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.53ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

UniProt accession

P30368

MASS（Da）

15,136

GeneID

101122781

Synonyms

B-cell stimulatory factor 1;BSF-1;IL-4;Interleukin-4;Lymphocyte stimulatory factor 1

Gene names

IL4

Research area

Immunology

Target protein

IL-4

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0079Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.105	2.002	1.951
	1.899
120ng/L	1.142	1.133	1.081
	1.123
60ng/L	0.451	0.436	0.385
	0.421
30ng/L	0.239	0.272	0.221
	0.306
15ng/L	0.148	0.152	0.101
	0.156
0ng/L	0.04	0.051	0
	0.063

Precision

We measured random samples of E0079Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	115.3	5.32	4.61
Intra-Assay	2	18	143.03	4.24	2.96
Intra-Assay	3	18	60.44	2.32	3.84

Protocols

> Protocol Booklet

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