The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha(IFNa).
Tested applications
ELISA
Reactivity
Sheep
Range
15.63-1000 pg/mL
Concentration
1000 pg/mL
Sensitivity
5.5 pg/mL
Protein Length
3.5h
Alternative names
IFNα1 ELISA Kit, IFL ELISA Kit, LeIF D ELISA Kit,
Note
For research use only
Application notes
Standard: 1000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IFNa in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
