Sheep Erythropoietin, EPO ELISA Kit
Cat# E0087Sh-5X96wells
Size : 5X96wells
Brand : Bioassay Technology Laboratory
Product overview
Full product name
Erythropoietin
Code
E0087Sh
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.048ng/mL
Detection range
0.1-40ng/mL
Sample type
Serum, plasma, cell culture supernates
Species
Sheep
Storage
2-8ºC
Assay time
1h 30m
Background
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
UniProt accession
MASS(Da)
21,335
GeneID
443302
Synonyms
EPO;Erythropoietin;MVCD2
Gene names
EPO
Research area
Cardiovascular; Signal Transduction; Stem Cells; Cancer
Target protein
EPO
Components
Components | Quantity |
---|---|
Pre-coated ELISA plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0087Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
24ng/mL | 1.689 | 1.742 | 1.711 |
1.795 | |||
12ng/mL | 1.005 | 1.02 | 0.989 |
1.034 | |||
6ng/mL | 0.632 | 0.585 | 0.554 |
0.537 | |||
3ng/mL | 0.267 | 0.203 | 0.172 |
0.139 | |||
1.5ng/mL | 0.073 | 0.107 | 0.076 |
0.141 | |||
0ng/mL | 0.032 | 0.031 | 0 |
0.03 |
Precision
We measured random samples of E0087Sh within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 14.32 | 0.67 | 4.7 |
Intra-Assay | 2 | 18 | 23.35 | 1.08 | 4.62 |
Intra-Assay | 3 | 18 | 7.81 | 0.38 | 4.89 |
Protocols
Product publications
Customer reviews and Q&As