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- Specifications
Product Description
Mouse monoclonal antibody raised against SARS-CoV-2 S.
Immunogen
SARS-CoV-2 VLP (Virus-like particle).
Host
Mouse
Reactivity
SARS-CoV-2
Form
Liquid
Purification
Protein A purification
Purity
> 98% (SDS-PAGE).
Isotype
IgG1, kappa
Recommend Usage
ELISA (1:5000-1:20000)
Flow Cytometry
Immunofluorescence(1:500-1:1000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 4°C for at least 6 months. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This monoclonal antibody is derived from SARS-CoV-2 VLP immune mouse.
- Applications
Western Blot (Transfected lysate)
Western Blot analysis of 293T cells were transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution.Western Blot (Recombinant protein)
Western Blot analysis of different recombinant proteins were stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) at 1:5000 dilution. Lane 1: SARS-CoV-2 S1-RBD, Lane 2: SARS-CoV-2 S1, Lane 3: SARS-CoV S1-RBD, Lane 4: SARS-CoV S1, and Lane 5: MERS-CoV S1-RBD.Immunofluorescence
Immunofluorescent staining of 293T cells were transfected with SARS CoV-2 spike plasmid and stained with SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) (1:1000).Enzyme-linked Immunoabsorbent Assay
ELISA assay of SARS-CoV-2 S monoclonal antibody, clone LGSV201 (Cat # MAB22570) to SARS-CoV-2 S1-RBD, SARS-CoV-2 S1, SARS-CoV S1, SARS-CoV S1-RBD, MERS-CoV S1-RBD and BSA (BSA: negative control).Flow Cytometry
- Publication Reference
- Efficient Inactivation of SARS-CoV-2 and Other RNA or DNA Viruses with Blue LED Light.
Chiara Terrosi, Gabriele Anichini, Jean Denis Docquier, Gianni Gori Savellini, Claudia Gandolfo, Francesco Saverio Pavone, Maria Grazia Cusi.
Pathogens 2021 Dec; 10(12):1590.
Application:WB, Virus, SARS-CoV-2 spike protein.
- Efficient Inactivation of SARS-CoV-2 and Other RNA or DNA Viruses with Blue LED Light.