RIP1 kinase inhibitor 1 [2095515-38-9]
Cat# HY-111409-10mg
Size : 10mg
Brand : MedChemExpress
RIP1 kinase inhibitor 1 (compound 22) is a highly potent, orally available, and brain-penetrating RIP1 kinase inhibitor (pKi=9.04).
For research use only. We do not sell to patients.
RIP1 kinase inhibitor 1 Chemical Structure
CAS No. : 2095515-38-9
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
View All RIP kinase Isoform Specific Products:
Description |
RIP1 kinase inhibitor 1 (compound 22) is a highly potent, orally available, and brain-penetrating RIP1 kinase inhibitor (pKi=9.04)[1]. |
IC50 & Target |
pKi: 9.04 (RIP1 kinase)[1] |
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In Vitro |
RIP1 kinase inhibitor 1 (compound 22) strongly suppresses necroptotic cell death and phosphorylation of MLKL(pMLKL) in human colorectal adenocarcinoma HT-29 cells (nectoptosis, IC50=2 nM; pMLKL, IC50=1.3 nM) as well as mouse L-cells NCTC 929 (nectoptosis, IC50=15 nM; pMLKL, IC50=2.7 nM)[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
461.90 |
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Formula |
C24H20ClN5O3 |
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CAS No. |
2095515-38-9 |
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Appearance |
Solid |
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Color |
White to yellow |
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SMILES |
N#CC1=CC=C2N(C)C([C@@H](N3CCC4=C(Cl)N(CC5=CC=CC=C5)N=C4C3=O)COC2=C1)=O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
DMSO : 200 mg/mL (432.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.96% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.1650 mL | 10.8249 mL | 21.6497 mL | 54.1243 mL |
5 mM | 0.4330 mL | 2.1650 mL | 4.3299 mL | 10.8249 mL | |
10 mM | 0.2165 mL | 1.0825 mL | 2.1650 mL | 5.4124 mL | |
15 mM | 0.1443 mL | 0.7217 mL | 1.4433 mL | 3.6083 mL | |
20 mM | 0.1082 mL | 0.5412 mL | 1.0825 mL | 2.7062 mL | |
25 mM | 0.0866 mL | 0.4330 mL | 0.8660 mL | 2.1650 mL | |
30 mM | 0.0722 mL | 0.3608 mL | 0.7217 mL | 1.8041 mL | |
40 mM | 0.0541 mL | 0.2706 mL | 0.5412 mL | 1.3531 mL | |
50 mM | 0.0433 mL | 0.2165 mL | 0.4330 mL | 1.0825 mL | |
60 mM | 0.0361 mL | 0.1804 mL | 0.3608 mL | 0.9021 mL | |
80 mM | 0.0271 mL | 0.1353 mL | 0.2706 mL | 0.6766 mL | |
100 mM | 0.0216 mL | 0.1082 mL | 0.2165 mL | 0.5412 mL |
RIP1 kinase inhibitor 1 Related Classifications
- Apoptosis
- RIP kinase