Reduced Glutathione (GSH) Colorimetric Assay Kit
Cat# E-BC-K030-S-100
Size : 100Assays
Brand : Elabscience
Detection Principle | Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm. |
Synonyms | GSH |
Sample Type | Serum,Plasma,Tissue,Cell |
Detection Method | Colorimetric method |
Detection Instrument | Spectrophotometer (420 nm) |
Research Area | Oxidative Stress , Cuproptosis |
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.26 mg GSH/L |
Detection Range | 0.26-122.8 mgGSH/L |
Precision | inter-assay CV: 2.4% | intra-assay CV: 1.8% |
Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
Human serum | 1 |
Mouse serum | 1 |
10% Mouse brain tissue homogenization | 1 |
10% Rat liver tissue homogenization | 1 |
Human plasma | 1 |
Rat plasma | 1 |
10% Carrot tissue homogenization | 1 |
293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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