Recombinant Rat Fractalkine (Chemokine Domain, aa 25-100)

Fractalkine (FKN) is a transmembrane mucinchemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴

The biological activity of Rat FKN was determined by its ability to chemoattract freshly isolated human peripheral blood lymphocytes (PBL). The expected ED₅₀ is 0.003 0.009 µg/ml.

Gln25 Gly100

Gln25

Lyophilized

The predicted molecular weight of Recombinant Rat Fractalkine is Mr 8.8 kDa.

8.8

This lyophilized protein is stable for six to twelve months when stored desiccated at 20°C to 70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Patel, DD. et al. (1998) J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424
4. Mori, M. et al. (2005) Int J Oncol. 26: 41