ICAM1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signaltransducing functions of ICAM1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

The biological activity of Mouse ICAM1 was determined by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to rmICAM coated plates (12.5 μg/ml with 100 μl/well), approximately 65 95% will adhere after PMA stimulation for 1 hour at RT.

Fc Fusion Protein

qvs ihpreaflpq ggsvqvncss sckedlslgl etqwlkdele sgpnwklfel seigedsspl cfencgtvqs sasatitvys fpesvelrpl pawqqvgkdl tlrchvdgga prtqlsavll rgeeilsrqp vgghpkdpke itftvlasrg dhganfscrt eldlrpqgla lfsnvsears lrtfdlpati pkldtpdlle vgtqqklfcs leglfpasea riylelggqm ptqestnssd svsatalvev teefdrtlpl rcvleladqi letqrtltvy nfsapvltls qlevsegsqv tvkceahsgs kvvllsgvep rpptpqvqft lnassedhkr sffcsaalev agkflfknqt lelhvlygpr ldetdclgnw twqegsqqtl kcqawgnpsp kmtcrrkadg allpigvvks vkqemngtyv chafsshgnv trnvyltvly hsqnniegrm dpkscdktht cppcpapell ggpsvflfpp kpkdtlmisr tpevtcvvvd vshedpevkf nwyvdgvevh naktkpreeq ynstyrvvsv ltvlhqdwln gkeykckvsn kalpapiekt iskakgqpre pqvytlppsr deltknqvsl tclvkgfyps diavewesng qpennykttp pvldsdgsff lyskltvdks rwqqgnvfsc svmhealhnh ytqkslslsp g

Gln28

Lyophilized

The predicted molecular weight of Recombinant Mouse ICAM1 is Mr 76.8 kDa. However, the actual molecular weight as observed by migration on SDSPAGE is Mr 110130 kDa.

76.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives present.

This lyophilized protein is stable for six to twelve months when stored desiccated at 20°C to 70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

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2. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
3. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
4. Ozcan, U. et al. (2009) Arch Gynecol Obstet.