Recombinant Human Interleukin-33

IL33 is a 30 kD proinflammatory cytokine that belongs to the IL1 superfamily. It signals inflammation in the body via the stimulation of macrophages, neutrophils, B cells, Th2 cells, eosinophils, basophils and mast cells. IL33 is a ligand for IL33R (ST2), which is part of a cytokine receptor gene cluster that includes IL1RI and IL1RII. IL33 binds ST2 and complexes with the IL1R Accessory Protein (IL1RAcP) to form a functional signaling receptor complex facilitating the activation of NFB and MAP kinase signaling pathways resulting in the production of type 2 cytokines from polarized Th2 cells. IL33R can exist as a type I transmembrane glycoprotein or a soluble glycoprotein. The soluble IL33R is thought to serve as a decoy receptor which competitively antagonizes the binding of IL33 thereby reducing its biological effects. IL33 has been linked with asthma, allergies, endometriosis, and hay fever. In addition, IL33 resides in keratinocytes of the skin and is thought to trigger the itching associated with dermatitis when subjected to irritation or allergic conditions. Furthermore, in APP/PS1 mice, IL33 has been shown to be effective in reversing the buildup and preventing the new formation of amyloid plaques that are characteristic of Alzheimer’s disease. However, the action of extracellular IL33 is limited due to rapid oxidation that causes a molecular conformational change which prevents it from binding to the ST2 receptor.

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Ser112

Lyophilized

The predicted molecular weight of Recombinant Human IL33, NFHEV, DVS 27 is Mr 18 kDa.

18

This recombinant protein has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 6.5 containing 50μg BSA per 1 ug as a carrier protein.

This lyophilized protein is stable for six to twelve months when stored desiccated at 20°C to 70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200400 µl of 1X PBS.