Recombinant Human G-CSF
Cat# G123-5
Size : 5µg
Brand : Leinco Technologies
Recombinant Human GCSF
Recombinant Human GCSF
Product No.: G123
Alternate Names Granulocyte Colony Stimulating Factor, CSF3, MGI1G, GMCSF Beta, Pluripoietin Product Type Recombinant Protein Expression Host E. coli Cells Species Human |
Data
BackgroundGranulocyte colonystimulating factor (GCSF), also known as CSF3, is an essential cytokine belonging to the CSF family of hormonelike glycoproteins that regulate haematopoietic cell proliferation and differentiation (1). It is produced primarily by monocytes and macrophages upon activation by endotoxin, TNFα and IFNγ but can be secreted by other cell types after LPS or IL1 activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express GCSF constitutively (2). The cell surface receptor for GCSF is GCSFR, also known as CD114. GCSF synergizes with some other cytokines, including GMCSF and IL4. Bone marrow is stimulated by GCSF to produce granulocytes and stem cells. GCSF is also a potent inducer of hematopoietic stem cell mobilization from the bone marrow into the bloodstream and therefore used clinically to facilitate hematopoietic recovery after bone marrow transplantation. GCSF also acts as a neurotrophic factor as its receptor is expressed by neurons in the brain and spinal cord. The action of GCSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis (3). These proprieties are currently under investigations for the development of treatments of neurological diseases such as cerebral ischemia. Human and murine GCSF are crossspecies reactive. Protein DetailsPurity >97% by SDSPAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human GCSF was determined in a cell proliferation assay using a murine myeloblastic cell line, NFS60 (Shirafuji, N. et al., 1989, Exp. Hematol. 17:116). The expected ED<sub>50</sub> for this effect is typically 10 60 pg/ml. Protein Accession No. Amino Acid Sequence tplgpasslp qsfllkcleq vrkiqgdgaa lqeklcatyk lchpeelvll ghslgipwap lsscpsqalq lagclsqlhs glflyqgllq alegispelg ptldtlqldv adfattiwqq meelgmapal qptqgampaf asafqrragg vlvashlqsf levsyrvlrh laqp Nterminal Sequence Analysis Met State of Matter Solution Predicted Molecular Mass The predicted molecular weight of Recombinant Human GCSF is Mr 18.8 kDa. However, the actual molecular weight as observed by migration on SDSPAGE is 18.5 kDa (reducing conditions) Predicted Molecular Mass 18.8 Formulation This recombinant protein solution was 0.2 µm filtered and formulated in Acetic Acid. Storage and Stability This protein is stable for three months when stored at 2°C to 8°C. DO NOT FREEZE. Loss of activity of >60% will be observed if this protein frozen. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day 28°C NCBI Gene Bank References & Citations1. KhannaGupta, A. et al. (2006) J. Leukoc. Biol. 79:1011 2. Nagata, S. et al. (1986) Nature 319:415 3. Demetri, GD. et al. (1991) Blood 78:2791 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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