Manual- Product Name
- Recombinant Canine TNFA
- Catalogue No.
- P0154
- Species
- Canine
- Host
- E.Coli
- Expression Region
- 77-233
- Mol. Weight
- 17.2 kDa
- Tags
- N-terminal His Tag
- Purity
- Greater than 90% as determined by SDS-PAGE.
- Endotoxin
- < 1 EU/μg as determined by LAL test.
- Application
- Western Blot, ELISA
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- APC1 protein Protein, Cachectin Protein, Cachetin Protein, DIF Protein, TNF Protein, TNF Protein, monocyte-derived Protein, tnfa Protein, tnf-a Protein, TNFalpha Protein, TNF-alpha Protein, TNF-alphacachectin Protein, TNFATNF Protein, macrophage-derived Protein, TNFSF1A Protein, TNFSF2 Protein, TNFSF2TNF superfamily Protein, member 2 Protein, tumor necrosis factor (TNF superfamily Protein, member 2) Protein
- SDS-PAGE
-