Recombinant Canine IFN gamma
Cat# RP0271D-005
Size : 5ug
Brand : Kingfisher Biotech
Interferon gammaIFNγ
IFNγ Homology Across Species
Canis lupus familiaris (dog) IFNγ – 100%
Canis lupus dingo (dingo) IFNγ – 100%
Vulpes vulpes (red fox) IFNγ – 100%
Nyctereutes procyonoides (raccoon dog) IFNγ – 100%
Phoca vitulina (harbor seal) IFNγ – 100%
Ailurus fulgens (lesser panda) IFNγ – 96%
More - https://blast.ncbi.nlm.nih.gov
Catalog No.:
RP0271D-005
Quantity:
5 ug
Source:
The Canine IFN gamma recombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Canine IFN gamma recombinant protein has a predicted molecular weight of 16.7 kDa.
Protein Sequence:
QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK (143)
Country of Origin:
USA
Applications:
The Canine IFN gamma endotoxin-free recombinant protein can be used in cell culture, as an IFN gamma ELISA Standard, and as a Western Blot Control.
Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells.
Osterbur K, Yu DH, Declue AE.
Res Vet Sci. 2012 Nov 8. doi:pii: S0034-5288(12)00305-0. 10.1016/j.rvsc.2012.10.002.
Applications: Stimulation of canine aortic endothelial cells
Abstract
The N-terminal portion of pro C-type natriuretic peptide (NT-pCNP) has shown promise as a biomarker for sepsis in humans and dogs, however the mechanism of NT-pCNP production in dogs is unknown. Canine aortic endothelial cells were stimulated with lipopolysaccharide, lipoteichoic acid, peptidoglycan, TNF-α, IL-1β, IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A or control (PBS), and NT-pCNP production was measured. Lipopolysaccharide, TNF-α, and IL-1β significantly stimulated NT-pCNP production in a dose and time dependent manner; IL-1β resulted in the greatest NT-pCNP concentrations. The other stimulants did not result in significant NT-pCNP production. The addition of serum to the cell culture model did not alter lipopolysaccharide, lipoteichoic acid or peptidoglycan induced NT-pCNP production. These data indicate that lipopolysaccharide, TNF-α and IL-1β regulate CNP production from canine vascular endothelium and of the stimulants tested, IL-1β is the predominant inducing factor. These data provide some initial insight into the mechanisms of CNP regulation in dogs.