Rat IgG (H&L) antibody (Peroxidase)
Cat# orb347862-1mg
Size : 1mg
Brand : Biorbyt
Rat IgG (H&L) antibody (Peroxidase)
Catalog Number: orb347862
Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (p/n orb347852). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (p/n orb347852) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.