RAT IgG (H&L) antibody (Peroxidase)
Cat# orb347858-2mg
Size : 2mg
Brand : Biorbyt
RAT IgG (H&L) antibody (Peroxidase)
Catalog Number: orb347858
ELISA results of Rabbit Anti-Rat IgG Antibody Peroxidase Conjugated tested against purified Rat IgG protein. Each well was coated in duplicate with 1.0 µg of Rat IgG [Green Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:41800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.
Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n orb347848). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (p/n orb347848) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.