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> RABBIT IgG (H&L) antibody (Biotin)

RABBIT IgG (H&L) antibody (Biotin)

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Cat# orb347769-2mg

Size : 2mg

Brand : Biorbyt


    RABBIT IgG (H&L) antibody (Biotin)

    Catalog Number: orb347769

    Catalog Numberorb347769
    CategoryAntibodies
    DescriptionRABBIT IgG (H&L) antibody (Biotin)
    Species/HostDonkey
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRabbit
    IsotypeIgG
    ImmunogenRabbit IgG whole molecule
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:300,000, IHC: 1:500 - 1:3000, WB: 1:3,000 - 1:15,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
    ConjugationBiotin
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesDonkey anti-Rabbit IgG Biotin Conjugated Antibody,
    NoteFor research use only
    Application notesAnti-Rabbit IgG (H&L) Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
    Expiration Date12 months from date of receipt.
    RABBIT IgG (H&L) antibody (Biotin)

    Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n orb347767). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody Peroxidase Conjugated (p/n orb347767) 1:5000 for 60 min at RT. Secondary antibody: None. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.