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> RABBIT IgG (H&L) antibody

RABBIT IgG (H&L) antibody

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Cat# orb347738-1mg

Size : 1mg

Brand : Biorbyt


    RABBIT IgG (H&L) antibody

    Catalog Number: orb347738

    Catalog Numberorb347738
    CategoryAntibodies
    DescriptionRABBIT IgG (H&L) antibody
    Species/HostSheep
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRabbit
    IsotypeIgG
    ImmunogenRabbit IgG whole molecule
    Concentration1.04 mg/mL
    Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
    ConjugationUnconjugated
    StorageStore vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesSheep Anti-Rabbit IgG Antibody Pre-Adsorbed, Sheep
    NoteFor research use only
    Application notesAnti-Rabbit IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    RABBIT IgG (H&L) antibody

    Western Blot of Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347744). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347744) 1:3000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n orb347953) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.