Images
Western Blot
Application

Western Blot

Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of IgG from different species with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • Specifications

    Product Description

    Goat recombinant monoclonal antibody raised against rabbit IgG Fc.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Goat

    Immunogen

    Original antibody is raised against rabbit IgG.

    Sequence

    N/A

    Reactivity

    Rabbit

    Specificity

    This antibody reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

    Form

    Liquid

    Purification

    Protein G purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.01 ug/mL- 0.5 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5 ug/mL-2 ug/mL)
    Immunohistochemistry (0.5 ug/mL-2 ug/mL)
    Immunoprecipitation
    Western Blot (0.1 ug/mL-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of IgG from different species with Rabbit IgG Fc monoclonal antibody, clone RMG02 (Cat # MAB12774) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

    Flow Cytometry