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> Rabbit IgG F(c) antibody (Biotin)

Rabbit IgG F(c) antibody (Biotin)

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Cat# orb347707-2mg

Size : 2mg

Brand : Biorbyt


    Rabbit IgG F(c) antibody (Biotin)

    Catalog Number: orb347707

    Catalog Numberorb347707
    CategoryAntibodies
    DescriptionRabbit IgG F(c) antibody (Biotin)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRabbit
    IsotypeIgG
    ImmunogenRabbit IgG F(c) fragment
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityAnti-RABBIT IgG F(c) (GOAT) was purified from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.
    ConjugationBiotin
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat anti-Rabbit IgG F(c) Antibody biotin Conjugat
    NoteFor research use only
    Application notesAnti-RABBIT IgG F(c) (GOAT) Biotin antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
    Expiration Date12 months from date of receipt.
    Rabbit IgG F(c) antibody (Biotin)

    Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n orb347661). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n orb347661) 1:2000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.