Rabbit IgG F(ab')2 antibody (Peroxidase)
Cat# orb347675-2mg
Size : 2mg
Brand : Biorbyt
Rabbit IgG F(ab')2 antibody (Peroxidase)
Catalog Number: orb347675
Western Blot of Anti-Rabbit IgG F(ab')2 (GOAT) Antibody (p/n orb535108). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab')2 (GOAT) Antibody (p/n orb535108) 1:10000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.