• Specifications

    Product Description

    Rabbit Fab polyclonal antibody raised against human IgG (H&L). The antibody is conjugated with Horseradish Peroxidase (HRP).

    Immunogen

    Human IgG whole molecule.

    Host

    Rabbit

    Reactivity

    Human

    Specificity

    IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).

    Form

    Lyophilized

    Conjugation

    HRP

    Purification

    This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

    Isotype

    IgG Fab

    Recommend Usage

    ELISA (1:10000-1:50000)
    Immunohistochemistry (1:500-1:2500)
    Western Blot (1:1000-1:10000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!

    Storage Instruction

    Store vial at 4°C prior to restoration.
    After reconstitution with 500 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

  • Applications

    Western Blot

    Immunohistochemistry

    Enzyme-linked Immunoabsorbent Assay