The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor(BDNF).
Tested applications
ELISA
Reactivity
Rabbit
Range
31.25-2000 pg/mL
Concentration
10 ng/mL
Sensitivity
13.4 pg/mL
Protein Length
3.5h
Alternative names
Neurotrophin ELISA Kit, Abrineurin ELISA Kit
Note
For research use only
Application notes
Standard: 2000 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit BDNF in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
