The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5(ADAMTS5).
Tested applications
ELISA
Reactivity
Rabbit
Range
0.16-10 ng/mL
Concentration
10 ng/mL
Sensitivity
0.059 ng/mL
Protein Length
3.5h
Alternative names
ADMP-2 ELISA Kit, Aggrecanase-2 ELISA Kit
Note
For research use only
Application notes
Standard: 10 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ADAMTS5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ADAMTS5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ADAMTS5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ADAMTS5 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types
Tissue homogenates, cell lysates and other biological fluids
