Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

Pyruvate kinase (PK) is an important enzyme involved in glycolysis metabolic pathway. PK catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, producing ATP and pyruvate. Lack of pyruvate kinase will cause the disease pyruvate kinase deficiency which slows down the process of glycolysis.

/587 nm) and color (λ = 570 nm). The increase in fluorescence intensity or color is proportional to the increase in pyruvate amount. The kit can detect 0.1 mU/mL pyruvate kinase.