CheKine Micro Pyruvate Decarboxylase (PDC) Assay Kit is designed for detecting PDC activity in the sample.
Hazard Information
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Storage
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Alternative names
oxynitrilase; pyruvate decarboxylase
Note
For research use only
Application notes
The kit provides a simple method for detecting PDC activity in a variety of biological samples such as tissues, cell, serum, bacterial. The principle is that PDC catalyzes the decarboxylation of pyruvate to produce acetaldehyde, and alcohol dehydrogenase (ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has an absorption peak at 340 nm, but NAD+ does not. PDC activity is calculated by measuring the rate of decrease in light absorption at 340 nm.