Expression Systems’ Production Boost Additive (PBA) is a nutrient boost designed to increase protein expression by supporting the increased needs of insect cells infected by recombinant baculovirus. PBA is a serum-free, animal-free formulation that complements ESF 921 and ESF AF insect cell culture media and extends the late and very-late expression period of production by maintaining high culture viability. PBA is a cocktail of nutrients designed for supplementation at a rate of 1–10% at 6–36 hours post infection.

• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Extends culture period by maintaining cell viability
• Increases protein expression                
• Available in 100 mL or 1 L bottles—inquire for custom packaging

Fig. 1          Fig. 2

Tni Pro cells (Fig. 1) were infected with a recombinant baculovirus at an MOI of 1. PBA was added at 1% of the culture volume 18 hours post infection. Sf9 cells (Fig. 2) were infected at an MOI of 0.02. PBA was added at 1% or 5% of the culture volume 36 hours post infection. The recombinant protein was secreted into the culture supernatant and harvested daily. Protein expression levels were determined by protein specific ELISA.