Reagents and instruments for immunology, cell biology and molecular biology.

Primary Human Glomerular Microvascular Endothelial Cells (Set with CultureBoost-R 4CB-500-R) (put into culture upon receipt-R or store in Liquid Nitrogen)

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Cat# ACBRI 128 V-R

Size : 1x10^6cells/ml(frozen)

Brand : Cell Systems Corporation

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Primary Human Glomerular Microvascular Endothelial Cells Day 2 ACBRI 128

PRODUCT INFO
CITATIONS
TESTS
RESEARCH
DOCUMENTATION

Increase Biological Relevance with Human Primary Cells

Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).

Isolated from normal, healthy donor tissue
Available at Passage 3 (<12 cumulative population doublings)
Each vial contains approximately 1 x 10⁶ cells
Each vial will initiate a Passage 4 cell culture in a 75 cm² flask
Available in reserved lots to enhance consistency in your research program

Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.

ELEVATE RESEARCH CONSISTENCY
WITH RESERVED LOT PROGRAM

The Reserved Lot Program is designed to assist researchers in securing consistent supplies of human primary cells for their long-term research projects.

How the LOT Program Can Benefit You:

Reserve a Specific Number of Vials: You can reserve a set number of vials from a designated lot at no cost, ensuring that you have continued access to the same consistent cell lot throughout your research.
Request New Lots: Our LOT program allows you to request the generation of new lots, with up to 200 vials made specifically for your research needs. While we typically offer 1.00 x 10⁶ cells/vial, we can customize the cell count per vial if necessary to meet your requirements.

RESERVE YOUR LOT TODAY! Email us at customerservice@cell-systems.com or call 425-823-1010.

A Selection of Citations for ACBRI 128 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells

"Dipeptidyl peptidase-4 inhibitor linagliptin reduces urinary albumin excretion through the protection of glomerular endothelial function" Kondo et al. Kawasaki Medical Journal, 2021.

"Differential procoagulatory response of microvascular, arterial and venous endothelial cells upon inflammation in vitro" Brandtner et al. Thrombosis Research, 2021.

"Proteoglycans contribute to the functional integrity of the glomerular endothelial cell surface layer and are regulated in diabetic kidney disease" Khramova et al. Scientific Reports, 2021.

"Lowering the increased intracellular pH of human‐induced pluripotent stem cell‐derived endothelial cells induces formation of mature Weibel‐Palade bodies" Tiemeier et al. Stem Cell Reports, 2020.

"Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts" Cohen et al. Scientific Reports, 2020.

"Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation" Tiemeier et al. Stem Cell Reports, 2019.

"Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip" Musah et al. Nature Protocols, 2018.

"Brain microvascular endothelial cells exhibit lower activation of the alternative complement pathway than glomerular microvascular endothelial cells" Sartain et al. J Biological Chemistry, 2018.
"Tacrolimus Prevents von Willebrand Factor Secretion by Allostimulated Human Glomerular Endothelium" Beland et al. Am J Transplantation, 2018.
"Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip" Musah et al. Nature and Biological Engineering, 2017.

"The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating" Pajecka et al. Experimental Cell Research, 2017.
"The human kidney capsule contains a functionally distinct mesenchymal stromal cell population" Leuning et al. PLoS One, 2017.
"Nitrite Activates 5′AMP-Activated Protein Kinase-Endothelial Nitric Oxide Synthase Pathway in Human Glomerular Endothelial Cells" Miyamoto et al. Biol. & Pharma Bulletin, 2017.
"Circulating Fibroblast Growth Factor-2, HIV-Tat, and Vascular Endothelial Cell Growth Factor-A in HIV-Infected Children with Renal Disease Activate Rho-A and Src in Cultured Renal Endothelial Cells" Das et al. PLoS One, 2016.
"TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human GLomerular Endothelial Cells" Sartain et al. J Immunology, 2016.
"TNF causes changes in glomerular endothelial permeability and morphology through a Rho and myosin light chain kinase-dependent mechanism" Xu et al. Physiological Reports, 2015.
"Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings" Turner and Moake, PLoS One, 2015.
"Adenosine Kinase Inhibition Protects The Kidney Against Streptozotocin-Induced Diabetes Through Anti-inflammatory and Anti-oxidant Mechanisms" Pye et al. Pharmacological Research, 2014.

Standard Tests

TESTS	RESULTS
HIV Serologic Test (donor level HIV AB EIA)	Negative
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab)	Negative
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level)	Negative

Retail Production (P3)Tests

TESTS	RESULTS
Bacterial Sterility (culture method) by independent lab	Pass
Fungal Sterility (culture method) by independent lab	Pass
Mycoplasma Sterility (culture method) by independent lab	Pass

Cell Markers and Functional Tests

TESTS	RESULTS
Inducible expression of CD 62E (E-Selectin)	> 90% positive by immunofluorescence
Cell adhesion protein CD31	> 95% positive by immunofluorescence
Cytoplasmic VWF / Factor VIII	> 95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL	> 95% positive by immunofluorescence

Immunofluorescence imagery and characterization in peer reviewed literature

ACBRI 128 at Passage 6, demonstrating uptake of fluorescently labeled DiI-Ac-LDL as functional test for endothelial cell identity.

Immunofluorescence staining showing expression of endothelial (EnC)-specific markers by human glomerular EnC (GEnC) cultured on coverslips. Magnification, x200.

Chart showing the effect of TNF-α on e-selectin expression by GEnC in a cell-based fluorescence immunoassay. GEnC were incubated with TNF-α at various concentrations or control medium for 6 h before fixing and labeling for e-selectin. E-selectin expression is proportional to fluorescence emission. Bars show mean ± SEM; n = 12; P < 0.001 by ANOVA.

Chart showing the results of separate experiments examining the effect of cAMP medium (containing 2 μM RO-20-1724 and 30 μM pCPT-cAMP) or thrombin (1 U/ml) on transendothelial electrical resistance (TEER) of GEnC monolayers after 1 h. Bars show mean ± SEM; n = 5, P = 0.006 for effect of cAMP; n = 8, P < 0.0005 for effect of thrombin, P values by t test. cAMP medium increased TEER by 34.4 Ω/cm² compared with control, whereas thrombin decreased TEER by 14.8 Ω/cm².

Graphs showing the effect of “cAMP medium” (containing 2 μM RO-20-1724 and 300 μM pCPT-cAMP; A) or thrombin (1 U/ml; B) on passage of FITC-BSA through GEnC monolayers over time. Points show mean ± SEM, n = 10, P = 0.003 for effect of cAMP; n = 10, P < 0.0005 for effect of thrombin by repeated measures [rm] ANOVA.

From Satchell et al (2004) J Am Soc Nephrology 15(3):566-574.

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