PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) PMAxx™
Cat# 31050-X
Size : 1kit
Brand : Biotium
Product Description
This kit is designed to perform viability PCR on E. coli. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the uidA gene.
Features
- Selectively detect viable E. coli using qPCR
- Contains primers to amplify the uidA gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
- Sufficient reagents for 200 PCR reactions
Kit Components
- PMA dye or PMAxx™ dye
- PMA Enhancer for Gram Negative Bacteria
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- uidA primer mix, 5 uM
About this kit
Escherichia coli is a species of bacteria that is commonly used in laboratories. Some strains of E. coli are food-borne pathogens and can cause digestive illness. For specific detection of the pathogenic E. coli strain O157:H7, please see kit number
31037
. PCR to amplify the gene uidA has been published and shown to be highly specific for E. coli. The primers provided in the kit have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.To learn more about the advantages of determining bacterial viability using viability PCR, visit the
Viability PCR Technology Page
.Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at
techsupport@biotium.com
.References
Download
PMA and PMAxx™ References
and a list ofPMA and PMAxx™ Validated Bacterial Strains
.