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> PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx™

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx™

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Cat# 31037-X

Size : 1kit

Brand : Biotium

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PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)
Viability dye

PMA, PMAxx™
Storage Conditions

Store at -10 to -35 °C
Assay type/options

Live/dead discrimination, Viability PCR
Detection method/readout

PCR/qPCR
Protocols

PI-Strain-Specific Bacterial Viability PCR Kits

SDS

SDS PMA Real-Time PCR Bacterial Viability Kits - Strain Specific

SDS PMAxx Real-Time PCR Bacterial Viability Kits - Strain Specific

Supporting Documents

PMA & PMAxx™ References

PMA & PMAxx™ Validated Bacterial Strains

PMAxx™ & PMA Flyer

Bacteria Stain Flyer

qPCR Master Mixes Flyer

Cell Viability and Cell Death Brochure

Product Description

This kit is designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the Z3276 gene.

Features

  • Selectively detect viable E. coli O157:H7 using qPCR
  • Contains primers to amplify the Z3276 gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • Z3276 primer mix, 5 uM

About this kit

E. coli O157:H7 is a pathogenic strain of Escherichia coli bacteria. Often a food-borne pathogen, it can cause digestive illness. PMA-based viability PCR to detect E. coli O157:H7 has been reported using the primers provided in the kit, and ORF Z3276 was shown to be specific for strain O157:H7 . These primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. For pan-E. coli primers to detect other strains of E. coli besides O157:H7, please see kit

31050

.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the

Viability PCR Technology Page

.

Strain-Specific Bacterial Viability PCR Kits

Bacteria Strain Gene name Kit with PMA (catalog #) Kit with PMAxx™ (catalog #)
Salmonella enterica invA

31033

31033-X
Mycobacterium tuberculosis groEL2

31034

 N/A
Staphylococcus aureus nuc

31035

 N/A
MRSA mecA

31036

 N/A
E. coli O157:H7 Z3276

31037

31037-X
E. coli uidA

31050

31050-X
Listeria monocytogenes hly

31051

31051-X
Legionella pneumophila mip

31053

 N/A

Don’t see your favorite strain? Let us know at

techsupport@biotium.com

.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Download

PMA and PMAxx™ References

and a list of

PMA and PMAxx™ Validated Bacterial Strains

.

 

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