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pLenti-P2A-Puro Lentiviral Gene Expression Vector

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Size : 10ug

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SKU

PS100109

pLenti-P2A-Puro, tag free Lenti vector with P2A-Puro, 10 ug

Datasheet SDS Vector Sequence

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Specifications
Product Data
E. coli Selection	Chl (34 ug/mL)
Mammalian Cell Selection	Puromycin
Expression Promoter	CMV
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34 ug/ml) V2_F Primer can be used to sequence the target sequence after cloning. P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Ambient

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100109

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

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