View product citations for antibody PAB15330 on CiteAb
  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of PDK1.PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide corresponding to residues within 350-436 at C-terminu of human PDK1.

    Host

    Rabbit

    Reactivity

    Human

    Specificity

    By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

    Form

    Liquid

    Conjugation

    DyLight 650

    Purification

    Immunogen affinity purification

    Recommend Usage

    Immunocytochemistry/Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 50 mM sodium borate (0.05% sodium azide).

    Storage Instruction

    Store at 4°C in the dark.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunocytochemistry

    Immunofluorescence

  • Gene Info — PDK1

    Entrez GeneID

    5163

    Protein Accession#

    Q15118

    Gene Name

    PDK1

    Gene Alias

    -

    Gene Description

    pyruvate dehydrogenase kinase, isozyme 1

    Omim ID

    602524

    Gene Ontology

    Hyperlink

    Gene Summary

    Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq

    Other Designations

    mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1

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  • Diseases