PAX4 Antibody
Cat# orb1822966-100ug
Size : 100ug
Brand : Biorbyt
PAX4 Antibody
Catalog Number: orb1822966
| Catalog Number | orb1822966 |
|---|---|
| Category | Antibodies |
| Description | Pax-4 (paired box gene 4) protein influences normal differentiation of Insulinproducing beta cells and influences normal pancreatic islet development. Pax-4 protein is a transcriptional repressor that binds to a common cis element in the Glucagon, Insulin and Somatostatin promoters. Mouse Pax-4 transcript is present in pancreatic islets, and the islet beta cell lines MIN6, beta TC and NIT1. Differentiation of endoderm-derived endocrine pancreas is mediated through Pax-4 and Pax-6. Pax-4 may act as a Pax-6 repressor due to the competition for binding sites and lower transactivation potential of Pax-4. The human Pax-4 gene encodes a deduced 350 amino acid protein that is 80% identical to the deduced mouse Pax-4 protein. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | PAX4/7599 |
| Tested applications | IHC-P |
| Reactivity | Human |
| Isotype | Mouse IgG1, kappa |
| Immunogen | A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the Paired box 4 antibody. |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
| Conjugation | Unconjugated |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Hazard Information | This Paired box 4 antibody is available for research use only. |
| UniProt ID | O43316 |
| Storage | Aliquot the Paired box 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human ovarian carcinoma tissue with Paired box 4 antibody (clone PAX4/7599). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.