This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Leukotriene A4(LTA4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukotriene A4(LTA4).
Tested applications
ELISA
Reactivity
All
Range
156.25-10000 pg/mL
Concentration
10000 pg/mL
Sensitivity
46.3 pg/mL
Protein Length
2h
Alternative names
LT-A4 ELISA Kit
Note
For research use only
Application notes
Standard: 10000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LTA4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LTA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LTA4 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Types
serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
