This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Epitestosterone(ET) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epitestosterone(ET).
Tested applications
ELISA
Reactivity
All
Range
156.25-10000 pg/mL
Concentration
10000 pg/mL
Sensitivity
15.29 pg/mL
Protein Length
2h
Alternative names
ET
Note
For research use only
Application notes
Standard: 10000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ET protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ET. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ET in the samples is then determined by comparing the OD of the samples to the standard curve
