Nitric Oxide (NO) Colorimetric Assay Kit (Nitrate Reductase Method)
Detection principle
Nitric oxide (NO) has active chemical properties and is quickly metabolized into NO2- and NO3- in the body, while NO2- is further transformed into NO3-. This method uses nitrate reductase specifically to reduce NO3- to NO2-, and the concentration of NO is measured by the color depth.
Performance characteristics
| Synonyms | NO |
| Sample type | serum, plasma, urine, tissue,cell and supernatant of cell samples |
| Sensitivity | 1.38 μmol/L |
| Detection range | 1.38-40 μmol/L |
| Assay type | Quantitative |
| Assay time | 100 min |
| Precision | Average inter-assay CV: 8%Average intra-assay CV: 6% |
| Other instruments required | Centrifuge, Vortex mixer, 37℃ Incubator |
| Storage | -20℃ |
| Valid period | 12 months |
Ralated product
| Cat Number | Product Name |
|---|---|
| E-BC-K001-M | Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method) |
| E-BC-K019-M | Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method) |
| E-BC-K025-M | Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method) |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits