NeoDye DNA Blue

Features
▪ Detects double-strand DNA and single-stranded RNA effectively.
▪ Offers a safe alternative to ethidium bromide staining.
▪ Exhibitssensitivity on par with EtBr.
▪ Non-toxic, non-mutagenic, and non-carcinogenic composition.
▪ Produces no hazardous waste, ensuring environmental friendliness.

Description

NeoDye DNA Blue is a sensitive, non-mutagenic and safer to the environment greenfluorescent DNA gel dye designed to replace the highly toxic ethidium bromide (EtBr)

forstaining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The sensitivity of NeoDye DNA Blue is much higher than NeoDye DNA orange and EB, and destaining is not required

Storage

Atroomtemperature protected from lightfortwo years

Highlights

Nontoxic: Unique lipophilic and macromolecular propertie make it incapable of penetrating cell membranes. Ames test also shows
the mutagenicity of NeoDye DNA Blue is far less than that of EB, and readily biodegradable, non-carcinogenic.
• Highly sensitive: Suitable for staining fragments of different sizes in electrophoresis gel.
• Highly stable: Suitable for using microwave or other heating methods to prepare agarose gel; extremely stable at room
temperature under acidic or alkaline conditions; highly resistant to light.
• High signal-to-noise ratio: Strong fluorescence signal of the sample with low background signal
• Simple operation: Similar to ethidium bromide, the dye does not degrade during the process of preparing gel or electrophoresis. It
only takes 30 minutes for staining after electrophoresis, and the fragment can be visualized by a UV-transilluminator directly without
detaining or washing.
• A broad range of applications: Applicable for precast protocol (add dye during gel preparation) and post-stain protocol (submerge
the gel in the staining solution); suitable for agarose gels and polyacrylamide gels electrophoresis; suitable for staining dsDNA, ssDNA
or RNA.
• Existing imaging system: NeoDye DNA Blue has an optimal excitation in the UV region around 474 nm. It is recommended to try blue
light for excitation observation. Standard UV-transilluminator used for EB staining observation can also be used.

Components

FOR RESEARCH USE ONLY