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> n-Octyl β-D-glucopyranoside [29836-26-8]

n-Octyl β-D-glucopyranoside [29836-26-8]

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Cat# HY-116285-500mg

Size : 500mg

Brand : MedChemExpress


n-Octyl-β-d-glucopyranoside is a non-ionic detergent, it can be widely used in the research of biotechnical, biochemical applications, solubilization and crystallization of membrane proteins. n-Octyl-β-d-glucopyranoside can completely inhibit cavitation-induced cell lysis in vitro.

For research use only. We do not sell to patients.

n-Octyl β-D-glucopyranoside Chemical Structure

n-Octyl β-D-glucopyranoside Chemical Structure

CAS No. : 29836-26-8

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of n-Octyl β-D-glucopyranoside:

  • n-Octyl β-D-glucopyranoside-d24 In-stock
  • 1-O-n-Octyl-β-D-glucopyranoside-d17 Get quote
Description

n-Octyl-β-d-glucopyranoside is a non-ionic detergent, it can be widely used in the research of biotechnical, biochemical applications, solubilization and crystallization of membrane proteins. n-Octyl-β-d-glucopyranoside can completely inhibit cavitation-induced cell lysis in vitro[1][2][3].

In Vitro

Suspensions of HL-60 cells are exposed to 1.057 MHz unfocused ultrasound for 5-15 s with various additions of alkyl glucopyranosides. 2 mM n-Octyl β-D-glucopyranoside (OGP) added to the medium resultes in 100% survival of the cells after 5 s exposure under conditions which produces 35%-100% cell lysis without the additive[3].
Variation of the concentration of n-Octyl β-D-glucopyranoside for 0.5 MPa exposure produced increased cavitation and lysis at 1 mM relative to 0 mM, but decreased cavitation at 5 mM[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

n-Octyl β-D-glucopyranoside Related Antibodies
Molecular Weight

292.37

Formula

C14H28O6
CAS No.

29836-26-8
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1OCCCCCCCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 116.67 mg/mL (399.05 mM; Need ultrasonic)

DMSO : 100 mg/mL (342.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4203 mL 17.1016 mL 34.2032 mL
5 mM 0.6841 mL 3.4203 mL 6.8406 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (244 KB) HNMR (133 KB) OR (518 KB)

Handling Instructions (2659 KB)
References

  • [1]. Konidala P, et al. Molecular dynamics characterization of n-octyl-beta-D-glucopyranoside micelle structure in aqueous solution. J Mol Graph Model. 2006 Sep;25(1):77-86.  [Content Brief]

    [2]. Gould RJ, et al. Effects of octyl beta-glucoside on insulin binding to solubilized membrane receptors. Biochemistry. 1981 Nov 24;20(24):6776-81.  [Content Brief]

    [3]. Douglas L Miller, et al. The influence of octyl β-D-glucopyranoside on cell lysis induced by ultrasonic cavitation. J Acoust Soc Am. 2011 Nov;130(5):3482-8.  [Content Brief]

  • [1]. Konidala P, et al. Molecular dynamics characterization of n-octyl-beta-D-glucopyranoside micelle structure in aqueous solution. J Mol Graph Model. 2006 Sep;25(1):77-86.

    [2]. Gould RJ, et al. Effects of octyl beta-glucoside on insulin binding to solubilized membrane receptors. Biochemistry. 1981 Nov 24;20(24):6776-81.

    [3]. Douglas L Miller, et al. The influence of octyl β-D-glucopyranoside on cell lysis induced by ultrasonic cavitation. J Acoust Soc Am. 2011 Nov;130(5):3482-8.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.4203 mL 17.1016 mL 34.2032 mL 85.5081 mL
5 mM 0.6841 mL 3.4203 mL 6.8406 mL 17.1016 mL
10 mM 0.3420 mL 1.7102 mL 3.4203 mL 8.5508 mL
15 mM 0.2280 mL 1.1401 mL 2.2802 mL 5.7005 mL
20 mM 0.1710 mL 0.8551 mL 1.7102 mL 4.2754 mL
25 mM 0.1368 mL 0.6841 mL 1.3681 mL 3.4203 mL
30 mM 0.1140 mL 0.5701 mL 1.1401 mL 2.8503 mL
40 mM 0.0855 mL 0.4275 mL 0.8551 mL 2.1377 mL
50 mM 0.0684 mL 0.3420 mL 0.6841 mL 1.7102 mL
60 mM 0.0570 mL 0.2850 mL 0.5701 mL 1.4251 mL
80 mM 0.0428 mL 0.2138 mL 0.4275 mL 1.0689 mL
100 mM 0.0342 mL 0.1710 mL 0.3420 mL 0.8551 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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n-Octyl β-D-glucopyranoside Related Classifications

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Keywords:

n-Octyl β-D-glucopyranoside29836-26-8Othersnon-ionic detergentbiotechnicalsolubilizationcrystallizationmembrane proteinInhibitorinhibitorinhibit