Msr1 rat monoclonal antibody, clone 2F8, Low Endotoxin
Specifications
Product Data
Clone Name
2F8
Application
ELISA, FC, FN, IHC, IP, WB
Recommended Dilution
ELISA. Western blot: Recognises CD204 in J774 cells under Non-Reduced Conditions only. Functional assays. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
RAW264 cell line.
Specificity
This antibody recognises the Murine Scavenger Receptor Class A (SRA), type I and II, also known as CD204.
CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.
Synonyms
MSR1, SCARA1
Note
Endotoxin Level: less than 0.01 EU/µg. Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
