Biotrend USA > Mouse Monoclonal Cytochrome c Antibody (7H8.2C12)
Mouse Monoclonal Cytochrome c Antibody (7H8.2C12)
Cat# TA336369
Size : 100ug
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Cytochrome C (CYCS) Mouse Monoclonal Antibody [Clone ID: 7H8.2C12]
| Product Data | |
| Clone Name | 7H8.2C12 |
|---|---|
| Application | FC, ICC/IF, IHC, Immunoblotting, WB |
| Recommended Dilution | Western Blot: 0.05-0.5 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Simple Western: 2.5 ug/ml, Immunohistochemistry-Paraffin: 1:10-1:500, Flow Cytometry: 1:20-1:2000, Immunohistochemistry: 1:10-1:500, Flow (Intracellular)), Flow (Cell Surface), Immunoblotting |
| Reactivity | Canine, Drosophila, Equine, Human, Mammalian, Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2b, kappa |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA result |
| Buffer | PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration | lot specific |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | cytochrome c, somatic |
| Database Link | |
| Background | In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3. |
| Synonyms | CYC; HCS; THC4 |
| Note | Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al. |
| Reference Data | |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Small cell lung cancer, Viral myocarditis |
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