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Mouse Monoclonal Anti-TIMP2 (Tissue Inhibitor of Metalloproteinase 2) [Clone 3A4]
Cat# NB-36-00788-P0
Size : 20ug
Brand : Neo Biotech
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TIMP2 (Tissue Inhibitor of Metalloproteinase 2) Antibody [3A4]
Catalog Number Formulation Size Price
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Formalin-fixed, paraffin-embedded human Placenta stained with TIMP2 Mouse Monoclonal Antibody (3A4)
SDS-PAGE Analysis of Purified TIMP2 Mouse Monoclonal Antibody (3A4). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebrafish
Research Areas
Cardiovascular, Immunology, AKT Signaling, Angiogenesis
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Secreted
Gene Name
TIMP2
Positive Control
Colon, Endometrial, HeLa cells. Normal Placenta. Breast, Prostate or Ovarian Carcinoma.
Immunogen
A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)
Alternate Names
Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinase 2
Functions
- Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Key References
- Murray et al. 1998. Gut. 43(6):791-7.
- Jeffery et al. 2009. Histopathology. 54(7):820-8
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2380196/
- https://pubmed.ncbi.nlm.nih.gov/2157214/
- https://pubmed.ncbi.nlm.nih.gov/8810321/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/2793861/
- https://pubmed.ncbi.nlm.nih.gov/1480041/
- https://pubmed.ncbi.nlm.nih.gov/2554304/
- https://pubmed.ncbi.nlm.nih.gov/1730286/
- https://pubmed.ncbi.nlm.nih.gov/8112602/
- https://pubmed.ncbi.nlm.nih.gov/1655733/
- https://pubmed.ncbi.nlm.nih.gov/11710594/
- https://pubmed.ncbi.nlm.nih.gov/7918391/
- https://pubmed.ncbi.nlm.nih.gov/9705310/
- https://pubmed.ncbi.nlm.nih.gov/9837731/
- https://pubmed.ncbi.nlm.nih.gov/12032297/
- https://pubmed.ncbi.nlm.nih.gov/24073280/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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