Mouse Monoclonal Anti-Keratin Pan, [Clone Lu-5]
Cat# 610145
Size : 50µg
Brand : Progen
Quantity | 50 µg |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG1 |
Conjugate | Unconjugated |
Application | ICC/IF, IHC |
Purification | Affinity chromatography |
Reactivity | Bovine, Chicken, Human, Mouse, Pleurodeles, Rat, Snake, Xenopus |
Storage before reconstitution | 2-8°C |
Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Clone | Lu-5 |
Immunogen | Cells of lung cancer cell line A549 and A2182 |
Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | Assay dependent |
Immunohistochemistry (IHC) - paraffin | 1:10-1:20 (protease treatment and/or microwave treatment recommended) |
Western Blot (WB) | Not applicable |
Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.
Polypeptide reacting: Most keratins present in human epithelia (including keratins - formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 - KK38) and basic (K81 - K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.
Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).
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