Mouse Monoclonal Anti-Desmoplakin 1/2, lyophilized, purified [Clone DP1 + 2-2.15]
Cat# 61003
Size : 50µg
Brand : Progen
Quantity | 50 µg |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG1 |
Conjugate | Unconjugated |
Application | ICC/IF, IHC, WB |
Purification | Affinity chromatography |
Reactivity | Bovine, Chicken, Human, Mouse, Rat |
Storage before reconstitution | 2-8°C until indicated expiry date |
Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Clone | DP1 + 2-2.15 |
Immunogen | Bovine desmoplakin 1 + 2 |
Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
UniprotID | A0A3Q1MR22 (Bovine), P15924 (Human), E9Q557 (Mouse), F1LMV6 (Rat) |
Synonym | Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP |
Tested applications | Tested dilutions |
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Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
Immunohistochemistry (IHC) - frozen | 1:10-1:50 |
Immunohistochemistry (IHC) - paraffin | 1:10-1:50 (microwave treatment recommended) |
Western Blot (WB) | Assay dependent |
DP1 + 2-2.15 shows distinct punctate membrane staining of different epithelia. Tumors specifically detected: primary and metastatic carcinoma and meningioma. Polypeptides reacting: Desmoplakin 1 and 2 (Mr 250,000 and 215,000).
The epitope recognized by clone DP1 + 2-2.15 is mapped to the N-terminus of the rod domain (Bornslaeger, E. A., 1996).
Bornslaeger, E. A. Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly. J. Cell Biol. 134, 985–1001 (1996).
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