Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000.
Reactivity
Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Mouse IgG3 γ3 chain.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG. Maximum Cross Reactivity: X Reactivity: Limit (Actual) Mouse IgG1: < /= 2% (1.0%) Mouse IgG2a: < /= 2% (1.0%) Mouse IgG2b: < /= 2% (1.0%) Mouse IgG3: 100% (100%) Mouse IgM: < /= 10% (7.7%)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction.
Presentation State
Aff - Purified
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
Unconjugated
Storage
Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.