Images
Western Blot
Application

Western Blot

Western Blot analysis of nonreduced(-) and reduced(+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161) at the following concentrations: 200 ng/mL, 50 ng/mL and 10 ng/mL. The result shows this antibody reacts to the C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against of mouse IgG2c.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic peptide corresponding to mouse IgG2c.

    Sequence

    N/A

    Reactivity

    Mouse

    Specificity

    Recognizes the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.01-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    Immunoprecipitation
    Western Blotting (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western Blot analysis of nonreduced(-) and reduced(+) mouse IgG2c with Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161).

    Immunohistochemistry

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse IgG2c monoclonal antibody, clone RM223 (Cat # MAB15161) at the following concentrations: 200 ng/mL, 50 ng/mL and 10 ng/mL. The result shows this antibody reacts to the C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.