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> MOUSE IgG2b antibody (ATTO 647N)

MOUSE IgG2b antibody (ATTO 647N)

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Cat# orb347473-500ug

Size : 500ug

Brand : Biorbyt


    MOUSE IgG2b antibody (ATTO 647N) [Out of stock]

    Catalog Number: orb347473

    Catalog Numberorb347473
    CategoryAntibodies
    DescriptionMOUSE IgG2b antibody (ATTO 647N)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsFC, FLISA, IF, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG2b heavy chain
    Concentration1.0 mg/mL
    Dilution rangeFLISA: >1:20,000, FC: 1:500 - 1:2,500, IF: >1:5,000, WB: 1:4,000 - 1:20,000
    Form/AppearanceLyophilized
    PurityAnti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationATTO-647N
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-MOUSE IgG2b Antibody ATTO647N Conjugatio
    NoteFor research use only
    Application notesAnti-Mouse IgG2b ATTO 647N Antibody has been tested by western blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Mouse IgG2b secondary antibody is available in a variety of formats. ATTO dye conjugated antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Expiration Date12 months from date of receipt.
    MOUSE IgG2b antibody (ATTO 647N) [Out of stock]

    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

    MOUSE IgG2b antibody (ATTO 647N) [Out of stock]

    Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG2b (gamma 2b chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG2b. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2b. Other band(s): none.