Mouse EPHB3 (Ephrin Type B Receptor 3) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Ephb3
Gene ID:	13845
Uniprot ID:	EPHB3_MOUSE
Sample Type:	tissue homogenates or other biological fluids.

Tissue Specificity	Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level). Expressed in myogenic progenitor cells.
Post Translational Modifications	Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-609 is required for interaction with SH2 domain-containing proteins. Ubiquitinated by RNF186, mainly through 'Lys-48' and 'Lys-63'-linked polyubiquitin chains.
Function	Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Protein Name	Ephrin Type-B Receptor 3 Developmental Kinase 5 Mdk-5 Tyrosine-Protein Kinase Receptor Sek-4
Database Links	Reactome: R-MMU-2682334 Reactome: -MMU-3928662 Reactome: -MMU-3928664 Reactome: -MMU-3928665
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Dendrite
Alternative ELISA Names	Ephrin Type-B Receptor 3 ELISA kit Developmental Kinase 5 ELISA kit Mdk-5 ELISA kit Tyrosine-Protein Kinase Receptor Sek-4 ELISA kit Ephb3 ELISA kit Etk2 ELISA kit Mdk5 ELISA kit Sek4 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance